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Modulation of protein expression of COMT and MRP1 by different treatments. A549 cells were treated with the indicated concentrations of EGCG and quercetin alone or in combination for 24h and 48h. COMT (A, B) and MRP1 (A, C) protein expression was evaluated by Western blot. Values are expressed as mean ± SD. The superscript letters represent significant difference between groups (P<0.05): a compared to vehicle control (NT); b compared to 40 μM of EGCG treatment; c compared to 20 μM of quercetin treatment.

Journal: Food & function

Article Title: Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo

doi: 10.1039/c2fo10254d

Figure Lengend Snippet: Modulation of protein expression of COMT and MRP1 by different treatments. A549 cells were treated with the indicated concentrations of EGCG and quercetin alone or in combination for 24h and 48h. COMT (A, B) and MRP1 (A, C) protein expression was evaluated by Western blot. Values are expressed as mean ± SD. The superscript letters represent significant difference between groups (P<0.05): a compared to vehicle control (NT); b compared to 40 μM of EGCG treatment; c compared to 20 μM of quercetin treatment.

Article Snippet: Membranes were incubated with rabbit anti-human COMT antibody (sc-25844) at a dilution of 1:1000 and MRP1 antibody (sc-7773-R, Santa Cruz, CA) at a dilution of 1:200 overnight at 4°C. β-actin protein was used as loading control.

Techniques: Expressing, Western Blot, Control